3ZK2
Crystal structure of the sodium binding rotor ring at pH 8.7
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-2 |
| Synchrotron site | ESRF |
| Beamline | ID23-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-10-29 |
| Detector | MARRESEARCH |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 135.770, 83.900, 150.990 |
| Unit cell angles | 90.00, 112.85, 90.00 |
Refinement procedure
| Resolution | 46.998 - 2.630 |
| R-factor | 0.2575 |
| Rwork | 0.255 |
| R-free | 0.29740 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3zk1 |
| RMSD bond length | 0.021 |
| RMSD bond angle | 2.333 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.800 |
| High resolution limit [Å] | 2.640 | 2.640 |
| Rmerge | 0.280 | 1.150 |
| Number of reflections | 92330 | |
| <I/σ(I)> | 6.99 | 1.57 |
| Completeness [%] | 98.3 | 90.8 |
| Redundancy | 4.2 | 3.96 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8.7 | pH 8.7 |
