3ZJP
M.acetivorans protoglobin in complex with imidazole
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-1 |
| Synchrotron site | ESRF |
| Beamline | ID14-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC CCD |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 79.970, 49.060, 51.430 |
| Unit cell angles | 90.00, 92.48, 90.00 |
Refinement procedure
| Resolution | 23.410 - 1.380 |
| R-factor | 0.16116 |
| Rwork | 0.159 |
| R-free | 0.19820 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2veb |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.270 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0110) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 23.410 | 1.450 |
| High resolution limit [Å] | 1.380 | 1.380 |
| Rmerge | 0.050 | 0.260 |
| Number of reflections | 40771 | |
| <I/σ(I)> | 13.1 | 3.4 |
| Completeness [%] | 98.9 | 97.8 |
| Redundancy | 2.3 | 2.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 0.4 M MONOBASIC AMMONIUM PHOSPHATE, 0.01 M POTASSIUM FERRICYANIDE, 0.04 M IMIDAZOLE |
