3ZJ7
Crystal structure of strictosidine glucosidase in complex with inhibitor-1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-04-24 |
| Detector | ADSC CCD |
| Spacegroup name | P 4 21 2 |
| Unit cell lengths | 159.219, 159.219, 110.974 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.730 - 2.500 |
| R-factor | 0.2584 |
| Rwork | 0.258 |
| R-free | 0.27567 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2jf7 |
| RMSD bond length | 0.023 |
| RMSD bond angle | 2.061 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | Auto-Rickshaw |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 2.630 | 2.630 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.120 | 0.330 |
| Number of reflections | 48795 | |
| <I/σ(I)> | 16.34 | 6.48 |
| Completeness [%] | 96.6 | 93.1 |
| Redundancy | 9.1 | 7.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 10% PEG4000, 0.3 M AMSO4, 0.1M NAOAC, PH 5.0 |
