3ZIX
Clostridium perfringens Enterotoxin with the N-terminal 37 residues deleted
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-2 |
| Synchrotron site | ESRF |
| Beamline | ID23-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-06-11 |
| Detector | MARRESEARCH |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 191.680, 128.290, 137.200 |
| Unit cell angles | 90.00, 133.81, 90.00 |
Refinement procedure
| Resolution | 47.030 - 1.900 |
| R-factor | 0.1775 |
| Rwork | 0.176 |
| R-free | 0.19700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2xh6 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.080 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.10.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.030 | 1.940 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.060 | 1.220 |
| Number of reflections | 189121 | |
| <I/σ(I)> | 17.4 | 1.6 |
| Completeness [%] | 98.2 | 95.1 |
| Redundancy | 7.7 | 7.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6 | 25 % PEG 1500, 0.1 M SPG BUFFER, PH 6.0 |
