3ZIN
Gu_alpha_helicase
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
Synchrotron site | Australian Synchrotron |
Beamline | MX2 |
Temperature [K] | 300 |
Collection date | 2012-07-31 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 79.094, 90.262, 100.704 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 19.830 - 2.000 |
R-factor | 0.1799 |
Rwork | 0.179 |
R-free | 0.19850 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1ial |
RMSD bond length | 0.010 |
RMSD bond angle | 0.940 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | MOLREP |
Refinement software | BUSTER (2.10.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 19.830 | 2.110 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.060 | 0.460 |
Number of reflections | 49123 | |
<I/σ(I)> | 10.3 | 6.5 |
Completeness [%] | 99.6 | 99.3 |
Redundancy | 7.1 | 14.55 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 6.5 | 0.8 M SODIUM CITRATE, 0.1 M HEPES BUFFER (PH 6.5) AND 10 MM DTT |