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3ZIN

Gu_alpha_helicase

Experimental procedure
Experimental methodSINGLE WAVELENGTH
Source typeSYNCHROTRON
Source detailsAUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron siteAustralian Synchrotron
BeamlineMX2
Temperature [K]300
Collection date2012-07-31
Spacegroup nameP 21 21 21
Unit cell lengths79.094, 90.262, 100.704
Unit cell angles90.00, 90.00, 90.00
Refinement procedure
Resolution19.830 - 2.000
R-factor0.1799
Rwork0.179
R-free0.19850
Structure solution methodMOLECULAR REPLACEMENT
Starting model (for MR)1ial
RMSD bond length0.010
RMSD bond angle0.940
Data reduction softwareXDS
Data scaling softwareSCALA
Phasing softwareMOLREP
Refinement softwareBUSTER (2.10.0)
Data quality characteristics
 OverallOuter shell
Low resolution limit [Å]19.8302.110
High resolution limit [Å]2.0002.000
Rmerge0.0600.460
Number of reflections49123
<I/σ(I)>10.36.5
Completeness [%]99.699.3
Redundancy7.114.55
Crystallization Conditions
crystal IDmethodpHtemperaturedetails
16.50.8 M SODIUM CITRATE, 0.1 M HEPES BUFFER (PH 6.5) AND 10 MM DTT

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