3ZII

Bacillus subtilis SepF G109K, C-terminal domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-1
Synchrotron site	ESRF
Beamline	ID23-1
Temperature [K]	100
Collection date	2011-09-04
Spacegroup name	C 2 2 21
Unit cell lengths	42.010, 80.140, 51.820
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	25.910 - 2.200
R-factor	0.1968
Rwork	0.195
R-free	0.24500
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	0.947
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX ((PHENIX.REFINE))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	51.820	2.320
High resolution limit [Å]	2.200	2.200
Rmerge	0.150	0.400
Number of reflections	4701
<I/σ(I)>	8.8	4.5
Completeness [%]	100.0	100
Redundancy	6.8	7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1				15% (V/V) ETHANOL, 0.1M TRIS PH 7.0