3ZIG

SepF-like protein from Pyrococcus furiosus

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-1
Synchrotron site	ESRF
Beamline	ID23-1
Temperature [K]	100
Detector technology	CCD
Collection date	2010-09-05
Detector	ADSC QUANTUM 315r
Spacegroup name	P 65 2 2
Unit cell lengths	59.327, 59.327, 177.484
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	29.581 - 2.500
R-factor	0.2223
Rwork	0.219
R-free	0.28880
Structure solution method	MAD
Starting model (for MR)	NONE
RMSD bond length	0.008
RMSD bond angle	0.973
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	SOLVE
Refinement software	PHENIX ((PHENIX.REFINE))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.580	2.640
High resolution limit [Å]	2.500	2.500
Rmerge	0.080	0.230
Number of reflections	7036
<I/σ(I)>	31.1	14.6
Completeness [%]	99.9	100
Redundancy	26.2	25.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1				0.2 M AMMONIUM SULFATE, 0.1 M HEPES PH 7.5, 25% W/V PEG 3350