3ZIE
SepF-like protein from Archaeoglobus fulgidus
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-09-09 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9793, 0.9798 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 107.020, 64.090, 82.640 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.000 |
| R-factor | 0.19073 |
| Rwork | 0.188 |
| R-free | 0.24416 |
| Structure solution method | MAD |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.025 |
| RMSD bond angle | 2.055 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 27.000 | 2.110 |
| High resolution limit [Å] | 1.960 | 2.000 |
| Rmerge | 0.070 | 0.110 |
| Number of reflections | 39136 | |
| <I/σ(I)> | 29.9 | 22.8 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 14 | 14.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 0.2 M LITHIUM SULFATE, 0.1 M SODIUM ACETATE PH 4.5, 30 %W/V PEG 8000 |
