3ZIB
Rap2a protein (SMA2265) from Serratia marcescens
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 287 |
| Detector technology | PIXEL |
| Collection date | 2012-09-22 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 39.650, 81.350, 58.450 |
| Unit cell angles | 90.00, 91.54, 90.00 |
Refinement procedure
| Resolution | 58.430 - 1.900 |
| R-factor | 0.18566 |
| Rwork | 0.183 |
| R-free | 0.23358 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4b6i |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.745 |
| Data reduction software | iMOSFLM |
| Data scaling software | SCALA (CCP4) |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.600 | 2.000 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.100 | 0.520 |
| Number of reflections | 29067 | |
| <I/σ(I)> | 15.3 | 4 |
| Completeness [%] | 99.4 | 98.9 |
| Redundancy | 7.4 | 7.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | SITTING DROP VAPOUR DIFFUSION. PROTEIN BUFFER: 10 MM SODIUM PHOSPHATE, PH 6.4 RESERVOIR CONDITION: 25% W/V POLYETHYLENE GLYCOL 1000, 0.1 M MES, PH6.5. CRYOPROTECTANT USED: 20% GLYCEROL WITH MOTHER LIQUOR. |
