3ZI5

Crystal STRUCTURE OF RESTRICTION ENDONUCLEASE BFII C-TERMINAL RECOGNITION DOMAIN IN COMPLEX WITH COGNATE DNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RUH3R
Temperature [K]	287
Detector technology	IMAGE PLATE
Collection date	2009-09-22
Detector	RIGAKU IMAGE PLATE
Spacegroup name	P 65
Unit cell lengths	175.180, 175.180, 35.789
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	43.795 - 3.200
R-factor	0.1805
Rwork	0.176
R-free	0.22590
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2C1L CHAIN A RESIDUES 200-358
RMSD bond length	0.003
RMSD bond angle	0.743
Data reduction software	MOSFLM
Data scaling software	TRUNCATE
Phasing software	MOLREP
Refinement software	PHENIX ((PHENIX.REFINE))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.570	3.230
High resolution limit [Å]	3.200	3.200
Rmerge	0.150	0.240
Number of reflections	10809
<I/σ(I)>	13.31	5.43
Completeness [%]	100.0	100
Redundancy	6.97	6.49

Crystallization Conditions

crystal ID	method	pH	temperature	details
1			292	CRYSTALS WERE GROWN AT 19C BY MIXING COMPLEX SOLUTION WITH THE CRYSTALLIZATION BUFFER 0.49 M NAH2PO4, 0.91 M K2HPO4 (PH6.9 AT 25C).