3ZI4
The structure of Beta-phosphoglucomutase Inhibited With Glucose-6-phosphate and Scandium Tetrafluoride
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-1 |
| Synchrotron site | ESRF |
| Beamline | ID14-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-11-20 |
| Detector | ADSC CCD |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 37.152, 54.238, 104.550 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.330 |
| R-factor | 0.13237 |
| Rwork | 0.130 |
| R-free | 0.17379 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2wf6 |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.676 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.400 |
| High resolution limit [Å] | 1.330 | 1.330 |
| Rmerge | 0.050 | 0.450 |
| Number of reflections | 46171 | |
| <I/σ(I)> | 18.7 | 2.4 |
| Completeness [%] | 94.0 | 76.6 |
| Redundancy | 3.4 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.2 | 19-21 % PEG 4000 AND 50 MM NAAC, pH 7.2 | ||
| 1 | 7.2 | 19-21 % PEG 4000 AND 50 MM NAAC, pH 7.2 |
