3ZI1
Crystal structure of human glyoxalase domain-containing protein 4 (GLOD4)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-10-18 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 21 2 21 |
| Unit cell lengths | 119.291, 42.918, 68.103 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 119.290 - 1.900 |
| R-factor | 0.16713 |
| Rwork | 0.165 |
| R-free | 0.20398 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1fa8 |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.463 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 68.100 | 2.000 |
| High resolution limit [Å] | 1.890 | 1.890 |
| Rmerge | 0.100 | 0.530 |
| Number of reflections | 27935 | |
| <I/σ(I)> | 8.9 | 2.2 |
| Completeness [%] | 98.2 | 97.4 |
| Redundancy | 4.4 | 4.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 0.2M MAGNESIUM CHLORIDE, 0.1M HEPES PH 7.5, 25%(W/V) PEG 3350 |
