3ZHP
Human MST3 (STK24) in complex with MO25beta
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04-1 |
| Synchrotron site | Diamond |
| Beamline | I04-1 |
| Temperature [K] | 100 |
| Collection date | 2011-12-07 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 64.800, 120.340, 98.910 |
| Unit cell angles | 90.00, 99.69, 90.00 |
Refinement procedure
| Resolution | 51.880 - 2.900 |
| R-factor | 0.2284 |
| Rwork | 0.227 |
| R-free | 0.26120 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB ENTRIES 3CKW 3GNI 1UPK 2WTK |
| RMSD bond length | 0.009 |
| RMSD bond angle | 0.990 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.11.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 63.880 | 3.060 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Rmerge | 0.070 | 0.780 |
| Number of reflections | 32108 | |
| <I/σ(I)> | 10 | 1.7 |
| Completeness [%] | 96.8 | 97.3 |
| Redundancy | 4.3 | 4.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 0.2M NA/KPO4, 10% PEG 3350, 10% ETHYLENE GLYCOL, pH 7.5 |
