3ZHN
Crystal structure of the T6SS lipoprotein TssJ1 from Pseudomonas aeruginosa
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 113 |
| Detector technology | IMAGE PLATE |
| Collection date | 2011-03-01 |
| Detector | RIGAKU RAXIS II |
| Spacegroup name | P 2 21 21 |
| Unit cell lengths | 38.315, 41.637, 76.947 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 22.750 - 1.400 |
| R-factor | 0.1633 |
| Rwork | 0.161 |
| R-free | 0.20187 |
| Structure solution method | SAD |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.020 |
| RMSD bond angle | 1.978 |
| Data reduction software | d*TREK |
| Data scaling software | SCALEPACK |
| Phasing software | SHELX |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.240 | 1.450 |
| High resolution limit [Å] | 1.400 | 1.400 |
| Rmerge | 0.096 | 0.460 |
| Number of reflections | 24682 | |
| <I/σ(I)> | 10.2 | 2.7 |
| Completeness [%] | 98.7 | 87 |
| Redundancy | 9.27 | 5.61 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 0.2M FORMATE, 15% PEG 5K MME |
