3ZHM

N-terminal domain of the CI repressor from bacteriophage TP901-1 in complex with the OL2 operator half-site

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-2
Synchrotron site	ESRF
Beamline	ID23-2
Temperature [K]	100
Detector technology	CCD
Detector	ADSC CCD
Spacegroup name	P 21 21 21
Unit cell lengths	29.860, 64.070, 67.820
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	33.910 - 2.600
R-factor	0.2009
Rwork	0.199
R-free	0.21840
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2r1j
RMSD bond length	0.002
RMSD bond angle	0.671
Data reduction software	MOSFLM
Phasing software	PHASER
Refinement software	PHENIX ((PHENIX.REFINE))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	33.910	2.740
High resolution limit [Å]	2.600	2.600
Rmerge	0.160	0.490
Number of reflections	4365
<I/σ(I)>	9	4.7
Completeness [%]	100.0	100
Redundancy	4.4	4.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		7		pH 7