3ZH6
The structure of Haemophilus influenzae Se_Met form of protein E
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | MAX II BEAMLINE I911-3 |
| Synchrotron site | MAX II |
| Beamline | I911-3 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-04-07 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.9700, 0.97918, 0.97942 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 44.085, 56.884, 61.315 |
| Unit cell angles | 90.00, 97.12, 90.00 |
Refinement procedure
| Resolution | 28.930 - 2.292 |
| R-factor | 0.1901 |
| Rwork | 0.187 |
| R-free | 0.25490 |
| Structure solution method | MAD |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.208 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | SHELX (SHARP) |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.000 | |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.090 | 0.500 |
| Number of reflections | 101090 | |
| <I/σ(I)> | 11.4 | 2.85 |
| Completeness [%] | 99.0 | 95.1 |
| Redundancy | 3.83 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 100 MM MES, PH 6, 200 MM NACL AND 20% PEG 6000 |
