3ZH0
Functional and structural role of the N-terminal extension in Methanosarcina acetivorans protoglobin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC CCD |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 80.670, 48.620, 92.030 |
| Unit cell angles | 90.00, 92.61, 90.00 |
Refinement procedure
| Resolution | 91.930 - 2.000 |
| R-factor | 0.20231 |
| Rwork | 0.199 |
| R-free | 0.26015 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2veb |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.469 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0110) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.600 | 2.100 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.190 | 0.480 |
| Number of reflections | 49973 | |
| <I/σ(I)> | 4.8 | 3.1 |
| Completeness [%] | 99.5 | 99.7 |
| Redundancy | 4 | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 0.2 M MAGNESIUM FORMATE |
