3ZGQ

Crystal structure of human interferon-induced protein IFIT5

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.3.1
Synchrotron site	ALS
Beamline	8.3.1
Temperature [K]	100
Detector technology	CCD
Detector	ADSC QUANTUM 315r
Spacegroup name	P 21 21 21
Unit cell lengths	63.833, 71.793, 116.920
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.704 - 2.203
R-factor	0.1821
Rwork	0.178
R-free	0.23980
Structure solution method	MAD
RMSD bond length	0.008
RMSD bond angle	1.121
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Refinement software	PHENIX ((PHENIX.REFINE))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.700	2.240
High resolution limit [Å]	2.200	2.200
Rmerge	0.110	0.700
Number of reflections	27780
<I/σ(I)>	14.6	2
Completeness [%]	99.9	100
Redundancy	4	4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		6.5		pH 6.5