3ZG8
Crystal Structure of Penicillin Binding Protein 4 from Listeria monocytogenes in the Ampicillin bound form
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PAL/PLS BEAMLINE 4A |
Synchrotron site | PAL/PLS |
Beamline | 4A |
Temperature [K] | 295 |
Detector technology | CCD |
Collection date | 2010-03-20 |
Detector | ADSC QUANTUM 315 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 84.746, 128.294, 54.732 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 29.698 - 2.094 |
R-factor | 0.1943 |
Rwork | 0.192 |
R-free | 0.23340 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3zg7 |
RMSD bond length | 0.010 |
RMSD bond angle | 1.080 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.180 |
High resolution limit [Å] | 2.100 | 2.100 |
Rmerge | 0.070 | 0.320 |
Number of reflections | 35362 | |
<I/σ(I)> | 27.3 | 4.5 |
Completeness [%] | 98.5 | 88.3 |
Redundancy | 5.5 | 3.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 7 | 20% POLYETHYLENE GLYCOL 3,350 0.1M AMMONIUM TARTRATE (PH 7.0) |