3ZFV
Crystal structure of an archaeal CRISPR-associated Cas6 nuclease
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-11-23 |
| Detector | ADSC QUANTUM 315r |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 71.650, 127.460, 83.620 |
| Unit cell angles | 90.00, 110.48, 90.00 |
Refinement procedure
| Resolution | 78.330 - 2.800 |
| R-factor | 0.20616 |
| Rwork | 0.205 |
| R-free | 0.23557 |
| Structure solution method | SAD |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.454 |
| Data reduction software | xia2 |
| Data scaling software | xia2 |
| Phasing software | PHENIX (AUTOSOLVE) |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 78.330 | 2.870 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.070 | 0.710 |
| Number of reflections | 34686 | |
| <I/σ(I)> | 23.3 | 3.7 |
| Completeness [%] | 99.9 | 99.1 |
| Redundancy | 10.4 | 10.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 9.1 | 0.05 M BICINE PH 9.1, 28 % PEG3350 |
