3ZFT
Crystal structure of product-like, processed N-terminal protease Npro at pH 3
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | BRUKER AXS MICROSTAR |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2011-06-03 |
| Detector | MARRESEARCH |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 41.830, 41.190, 44.370 |
| Unit cell angles | 90.00, 115.00, 90.00 |
Refinement procedure
| Resolution | 10.000 - 1.800 |
| R-factor | 0.19205 |
| Rwork | 0.188 |
| R-free | 0.26687 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3zfn |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.352 |
| Data reduction software | iMOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 10.000 | 1.900 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.080 | 0.980 |
| Number of reflections | 12285 | |
| <I/σ(I)> | 11.3 | 1.2 |
| Completeness [%] | 95.4 | 93.8 |
| Redundancy | 3.6 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 3.1 | 100MM NAACETATE, PH 8.5 50% PEG6000 |
