3ZFK
N-terminal truncated Nuclease Domain of Colicin E7
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | MAX II BEAMLINE I911-3 |
| Synchrotron site | MAX II |
| Beamline | I911-3 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-11-17 |
| Detector | MARMOSAIC 225 mm CCD |
| Spacegroup name | P 32 |
| Unit cell lengths | 55.400, 55.400, 73.200 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 29.099 - 1.700 |
| R-factor | 0.2017 |
| Rwork | 0.200 |
| R-free | 0.23430 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1m08 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.278 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | PHENIX ((PHENIX.REFINE: 1.8.2_1309)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.100 | 1.800 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.040 | 0.390 |
| Number of reflections | 27490 | |
| <I/σ(I)> | 28.7 | 10.5 |
| Completeness [%] | 97.3 | 93.4 |
| Redundancy | 2.9 | 2.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 4.5 | 0.1 M LITHIUM SULFATE, 50 MM SODIUM ACETATE PH 4.5, 25 % W/V PEG 400 |
