3ZFI
Rap1a protein (SMA2260) from Serratia marcescens
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 287 |
| Detector technology | CCD |
| Collection date | 2011-08-01 |
| Detector | ADSC QUANTUM 315r |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 82.650, 93.000, 51.260 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 61.780 - 1.980 |
| R-factor | 0.19416 |
| Rwork | 0.192 |
| R-free | 0.23396 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.925 |
| Data reduction software | iMOSFLM |
| Data scaling software | SCALA (CCP4) |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.500 | 2.090 |
| High resolution limit [Å] | 1.980 | 1.980 |
| Rmerge | 0.070 | 0.490 |
| Number of reflections | 14089 | |
| <I/σ(I)> | 13.4 | 2.7 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 4.5 | 4.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | SITTING DROP VAPOUR DIFFUSION. PROTEIN CONCENTRATION AT 9MG/ML, IN 150 MM SODIUM CHLORIDE, 25MM TRIS-HCL, PH 7.5 (SAMPLE BUFFER) RESERVOIR: 25% W/V POLYETHYLENE GLYCOL 3350, 0.1M BIS-TRIS, PH 5.5 2:1 SAMPLE TO RESERVOIR RATIO. |
