3ZF3
Phage dUTPases control transfer of virulence genes by a proto-oncogenic G protein-like mechanism. (Staphylococcus bacteriophage 80alpha dUTPase Y84I mutant).
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I04-1 |
Synchrotron site | Diamond |
Beamline | I04-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Detector | ADSC CCD |
Spacegroup name | P 21 3 |
Unit cell lengths | 87.180, 87.180, 87.180 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 61.650 - 3.100 |
R-factor | 0.23822 |
Rwork | 0.232 |
R-free | 0.27781 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3zez |
RMSD bond length | 0.004 |
RMSD bond angle | 0.783 |
Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 61.700 | 3.270 |
High resolution limit [Å] | 3.100 | 3.100 |
Rmerge | 0.080 | 0.300 |
Number of reflections | 4199 | |
<I/σ(I)> | 6.8 | 2.6 |
Completeness [%] | 99.9 | 100 |
Redundancy | 5 | 5.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 |