3ZET
Structure of a Salmonella typhimurium YgjD-YeaZ heterodimer.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC CCD |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 121.152, 61.663, 83.835 |
| Unit cell angles | 90.00, 101.14, 90.00 |
Refinement procedure
| Resolution | 34.380 - 2.310 |
| R-factor | 0.1811 |
| Rwork | 0.178 |
| R-free | 0.23470 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.030 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | BUSTER (2.11.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.380 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.120 | 0.650 |
| Number of reflections | 26090 | |
| <I/σ(I)> | 25.1 | 5.2 |
| Completeness [%] | 96.7 | 100 |
| Redundancy | 4 | 4.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8 | 20 GLYCEROL, 16 POLYETHYLENE GLYCOL 8000, 0.080 M SODIUM CACODYLATE PH 6.5, 0.160 M MAGNESIUM ACETATE, 20 MM CADMIUM CHLORIDE DIHYDRATE |
