3ZED

X-ray structure of the birnavirus VP1-VP3 complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04-1
Synchrotron site	Diamond
Beamline	I04-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2011-05-30
Detector	DECTRIS PILATUS 6M
Spacegroup name	P 21 21 2
Unit cell lengths	118.270, 341.870, 59.700
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	59.130 - 2.200
R-factor	0.181
Rwork	0.179
R-free	0.21450
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2yi9
RMSD bond length	0.010
RMSD bond angle	0.990
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	BUSTER (2.11.2)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	68.400	2.260
High resolution limit [Å]	2.200	2.200
Rmerge	0.120
Number of reflections	123796
<I/σ(I)>	12	1.2
Completeness [%]	99.7	99.2
Redundancy	6.7	6.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		7.5		20% (W/V) POLYETHYLENE GLYCOL 3350 AND 0.2M POTASSIUM FLUORIDE, pH 7.5