3ZEC
Fic protein from SHEWANELLA ONEIDENSIS (E73G mutant) in complex with AMPPNP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-10-29 |
| Detector | MARMOSAIC 225 mm CCD |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 71.320, 80.630, 141.780 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 14.993 - 2.200 |
| R-factor | 0.1645 |
| Rwork | 0.162 |
| R-free | 0.21050 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3eqx |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.192 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 42.660 | 2.300 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.100 | 0.530 |
| Number of reflections | 42250 | |
| <I/σ(I)> | 17.57 | 4.33 |
| Completeness [%] | 99.9 | 99.9 |
| Redundancy | 7.4 | 7.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.1 | 22% W/V PEG3350, 0.2M NAF PH 7.1 |
