3ZE2
Integrin alphaIIB beta3 headpiece and RGD peptide complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23ID |
Synchrotron site | APS |
Beamline | 23ID |
Temperature [K] | 100 |
Detector technology | CCD |
Detector | MARRESEARCH |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 233.210, 143.560, 104.730 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 54.018 - 2.350 |
R-factor | 0.1755 |
Rwork | 0.175 |
R-free | 0.20370 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3t3p |
RMSD bond length | 0.006 |
RMSD bond angle | 0.676 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.410 |
High resolution limit [Å] | 2.350 | 2.350 |
Rmerge | 0.140 | 0.940 |
Number of reflections | 146644 | |
<I/σ(I)> | 11 | 1.9 |
Completeness [%] | 100.0 | 99.8 |
Redundancy | 7.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 8.9 | 11% PEG 8000, 0.2 M AMMONIUM SULFATE, 0.1 M TRIS-HCL, PH 8.9 |