3ZDU
Crystal structure of the human CDKL3 kinase domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-10-29 |
| Detector | ADSC CCD |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 63.930, 63.930, 163.630 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 45.890 - 2.200 |
| R-factor | 0.21662 |
| Rwork | 0.214 |
| R-free | 0.26248 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | ENSEMBLE OF PDB ENTRIES 4AGU AND 4AAA |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.510 |
| Data reduction software | MOSFLM |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 54.540 | 2.270 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.100 | 0.630 |
| Number of reflections | 20446 | |
| <I/σ(I)> | 10.7 | 2.5 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 5.2 | 2.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 0.03M ZN CL, 30% PEG_6000, 5% ETHYLENE GLYCOL, MES PH5.5 |
