3ZDT
Crystal structure of basic patch mutant FAK FERM domain FAK31- 405 K216A, K218A, R221A, K222A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2010-11-18 |
| Detector | DECTRIS PILATUS |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 41.567, 175.527, 60.032 |
| Unit cell angles | 90.00, 93.94, 90.00 |
Refinement procedure
| Resolution | 43.880 - 3.150 |
| R-factor | 0.21745 |
| Rwork | 0.214 |
| R-free | 0.27822 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2al6 |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.609 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.340 |
| High resolution limit [Å] | 3.150 | 3.150 |
| Rmerge | 0.100 | 0.520 |
| Number of reflections | 14023 | |
| <I/σ(I)> | 12.5 | 2.6 |
| Completeness [%] | 99.6 | 99.6 |
| Redundancy | 3.77 | 3.76 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8.5 | 14% PEG4K, 200 MM MGCL2, 100 MM TRIS PH 8.5, 10 MM TCEP |
