3ZDP
R416A Monomeric nucleoprotein of influenza A virus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-02-05 |
| Detector | ADSC QUANTUM 315r |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 165.420, 285.440, 118.310 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 143.120 - 2.690 |
| R-factor | 0.20039 |
| Rwork | 0.198 |
| R-free | 0.24983 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2iqh |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.715 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.6.0116) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 10.000 | 2.820 |
| High resolution limit [Å] | 2.690 | 2.690 |
| Rmerge | 0.100 | 0.690 |
| Number of reflections | 77425 | |
| <I/σ(I)> | 11.75 | 1.91 |
| Completeness [%] | 99.4 | 99.7 |
| Redundancy | 3.3 | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 0.1 M HEPES PH 7.5, 1.2 M POTASSIUM SODIUM TARTRATE |
