3ZDO
Tetramerization domain of Measles virus phosphoprotein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-2 |
| Synchrotron site | ESRF |
| Beamline | ID23-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-11-20 |
| Detector | MARMOSAIC 225 mm CCD |
| Spacegroup name | P 1 |
| Unit cell lengths | 27.670, 45.720, 115.830 |
| Unit cell angles | 91.42, 93.58, 90.08 |
Refinement procedure
| Resolution | 8.000 - 2.070 |
| R-factor | 0.21332 |
| Rwork | 0.210 |
| R-free | 0.28397 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | AB-INITIO MODELS |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.453 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 8.000 | 2.150 |
| High resolution limit [Å] | 2.070 | 2.070 |
| Rmerge | 0.060 | 0.550 |
| Number of reflections | 32718 | |
| <I/σ(I)> | 9.27 | 1.6 |
| Completeness [%] | 94.7 | 88.5 |
| Redundancy | 2.2 | 2.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 5.6 | 100 MM TRI-SODIUM CITRATE PH 5.6, 20% MPD |
