3ZD9
Potassium bound structure of E. coli ExoIX in P21
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 |
| Temperature [K] | 298 |
| Detector technology | IMAGE PLATE |
| Detector | MARRESEARCH |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 53.472, 38.275, 59.750 |
| Unit cell angles | 90.00, 107.95, 90.00 |
Refinement procedure
| Resolution | 19.870 - 2.000 |
| R-factor | 0.16337 |
| Rwork | 0.159 |
| R-free | 0.24589 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3zd8 |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.421 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 2.050 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.090 | 0.370 |
| Number of reflections | 14512 | |
| <I/σ(I)> | 11.4 | 4.5 |
| Completeness [%] | 89.0 | 85.3 |
| Redundancy | 2.9 | 2.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.5 | 0.2 M SODIUM THIOCYANATE, 10MM ZNCL2 20% PEG 3350, 0.1 M MES AT PH 6.5 |
