3ZCX
Structure of GAPDH from Thermosynechococcus elongatus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-02-05 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 141.930, 141.930, 76.060 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 70.960 - 2.190 |
| R-factor | 0.16319 |
| Rwork | 0.161 |
| R-free | 0.20840 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3b1j |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.933 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 76.060 | 2.250 |
| High resolution limit [Å] | 2.190 | 2.190 |
| Rmerge | 0.090 | 0.630 |
| Number of reflections | 40432 | |
| <I/σ(I)> | 4.61 | 1.06 |
| Completeness [%] | 99.8 | 100 |
| Redundancy | 6.18 | 6.21 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 60 MM SODIUM CITRATE PH 5.5, 12% W/V PEG 3000 |
