3ZCU
Rabbit muscle glycogen phosphorylase b in complex with N-(pyridyl-2- carbonyl)-N-beta-D-glucopyranosyl urea determined at 2.05 A resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X13 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X13 |
| Temperature [K] | 298 |
| Detector technology | CCD |
| Collection date | 2007-02-14 |
| Detector | MARRESEARCH SX-165 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 128.414, 128.414, 116.523 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 24.080 - 2.050 |
| R-factor | 0.1867 |
| Rwork | 0.185 |
| R-free | 0.21588 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2qnb |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.004 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0072) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.090 |
| High resolution limit [Å] | 2.050 | 2.050 |
| Rmerge | 0.050 | 0.420 |
| Number of reflections | 61537 | |
| <I/σ(I)> | 21.2 | 4.1 |
| Completeness [%] | 99.0 | 99.1 |
| Redundancy | 5.3 | 5.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.8 | pH 6.8 |
