3ZCS
Rabbit muscle glycogen phosphorylase b in complex with N-(1-naphthoyl) -N-beta-D-glucopyranosyl urea determined at 2.07 A resolution
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | EMBL/DESY, HAMBURG BEAMLINE BW7B |
Synchrotron site | EMBL/DESY, HAMBURG |
Beamline | BW7B |
Temperature [K] | 298 |
Detector technology | CCD |
Collection date | 2002-07-05 |
Detector | ADSC CCD |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 128.283, 128.283, 116.513 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 29.500 - 2.030 |
R-factor | 0.19297 |
Rwork | 0.192 |
R-free | 0.21670 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2qnb |
RMSD bond length | 0.007 |
RMSD bond angle | 0.999 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | REFMAC (5.5.0072) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.060 |
High resolution limit [Å] | 2.030 | 2.030 |
Rmerge | 0.060 | 0.270 |
Number of reflections | 61970 | |
<I/σ(I)> | 15.1 | 6.7 |
Completeness [%] | 98.0 | 98.2 |
Redundancy | 4 | 4.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 6.8 | pH 6.8 |