3ZCN
Fic protein from SHEWANELLA ONEIDENSIS in complex with ATP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-08-14 |
| Detector | MARMOSAIC 225 mm CCD |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 37.790, 164.910, 70.230 |
| Unit cell angles | 90.00, 94.43, 90.00 |
Refinement procedure
| Resolution | 14.992 - 1.700 |
| R-factor | 0.1681 |
| Rwork | 0.166 |
| R-free | 0.20220 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3eqx |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.128 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 82.460 | 1.800 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.040 | 0.410 |
| Number of reflections | 93236 | |
| <I/σ(I)> | 22.78 | 3.46 |
| Completeness [%] | 99.5 | 97.7 |
| Redundancy | 3.9 | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.1 | 21%W/V PEG 3350, 0.2M NAF PH 7.1 |
