3ZCC
High resolution structure of the asymmetric R333G Hamp-Dhp mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | MARRESEARCH |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 36.060, 57.460, 52.340 |
| Unit cell angles | 90.00, 107.84, 90.00 |
Refinement procedure
| Resolution | 37.643 - 1.250 |
| R-factor | 0.1903 |
| Rwork | 0.190 |
| R-free | 0.20390 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3YRX |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.312 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | PHENIX ((PHENIX.REFINE: 1.8.1_1168)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 37.000 | 1.330 |
| High resolution limit [Å] | 1.250 | 1.250 |
| Rmerge | 0.040 | 0.650 |
| Number of reflections | 52485 | |
| <I/σ(I)> | 14.6 | 1.77 |
| Completeness [%] | 93.3 | 77.6 |
| Redundancy | 3.7 | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 |
