3ZC4
The structure of Csa5 from Sulfolobus solfataricus.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I24 |
| Synchrotron site | Diamond |
| Beamline | I24 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2011-02-16 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 285.000, 53.720, 216.680 |
| Unit cell angles | 90.00, 124.65, 90.00 |
Refinement procedure
| Resolution | 58.610 - 2.720 |
| R-factor | 0.20535 |
| Rwork | 0.205 |
| R-free | 0.21690 |
| Structure solution method | SAD |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.129 |
| Data reduction software | xia2 |
| Data scaling software | xia2 |
| Phasing software | PHENIX (AUTOSOLVE) |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 59.420 | 2.800 |
| High resolution limit [Å] | 2.720 | 2.720 |
| Rmerge | 0.110 | 0.810 |
| Number of reflections | 73275 | |
| <I/σ(I)> | 12.3 | 2.3 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 6.7 | 7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 4 | 0.1 M SODIUM ACETATE PH 4.0, 0.23 M POTASSIUM CHLORIDE AND 39 % PEG500. |
