3ZBU
Ferredoxin-NADP Reductase Mutant with SER 80 Replaced by ALA (S80A)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | BRUKER-INCOATEC MICROFOCUS |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-07-14 |
| Detector | BRUKER AXIOM |
| Spacegroup name | P 65 |
| Unit cell lengths | 86.985, 86.985, 96.322 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 75.380 - 1.890 |
| R-factor | 0.18262 |
| Rwork | 0.181 |
| R-free | 0.20461 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1que |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.278 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 59.530 | 1.990 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.090 | 0.260 |
| Number of reflections | 30565 | |
| <I/σ(I)> | 17.05 | 3.67 |
| Completeness [%] | 99.9 | 99.3 |
| Redundancy | 4.74 | 3.48 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 18 % PEG 6000, 0.1 M NAAC PH 5.5, 20 MM (NH4)2SO4 |
