3X3U

Crystal structure of wild-type of E. coli CutA1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL26B2
Synchrotron site	SPring-8
Beamline	BL26B2
Temperature [K]	100
Detector technology	CCD
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	1.0000
Spacegroup name	P 21 21 21
Unit cell lengths	62.419, 96.868, 106.398
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	38.270 - 2.090
R-factor	0.20845
Rwork	0.208
R-free	0.23800
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	1.217
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	REFMAC (5.7.0029)

Data quality characteristics

	Overall
Low resolution limit [Å]	38.270
High resolution limit [Å]	2.090
Number of reflections	36800
Completeness [%]	99.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		298	0.1M HEPES-Na pH7.5, 1.4M tri-Sodium Citrate dihydrate, VAPOR DIFFUSION, temperature 298.0K