3X3B
Crystal structure of the light-driven sodium pump KR2 in acidic state
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL32XU |
| Synchrotron site | SPring-8 |
| Beamline | BL32XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-10-16 |
| Detector | RAYONIX MX225HE |
| Wavelength(s) | 1.000000 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 40.582, 81.544, 233.775 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 40.561 - 2.300 |
| R-factor | 0.2107 |
| Rwork | 0.207 |
| R-free | 0.24860 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3ddl |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.005 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | MOLREP |
| Refinement software | PHENIX ((phenix.refine: 1.8.3_1479)) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.430 |
| High resolution limit [Å] | 2.300 | 6.780 | 2.290 |
| Number of reflections | 17741 | ||
| Completeness [%] | 97.8 | 96.1 | 96.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | LIPIDIC CUBIC PHASE | 4 | 293 | 42% PEG200, 0.1M sodium acetate pH 4.0, 0.05M MgCl2, lipidic cubic phase, temperature 293.0K |
