3X2R
Structure of the nonameric bacterial amyloid secretion channel CsgG
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U |
Synchrotron site | SSRF |
Beamline | BL17U |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-03-13 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.9792 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 171.877, 176.715, 103.524 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 48.071 - 2.900 |
R-factor | 0.2462 |
Rwork | 0.244 |
R-free | 0.29210 |
Structure solution method | SAD |
RMSD bond length | 0.007 |
RMSD bond angle | 1.302 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SOLVE |
Refinement software | PHENIX ((phenix.refine: 1.8_1069)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 3.000 |
High resolution limit [Å] | 2.900 | 2.900 |
Number of reflections | 61340 | |
Completeness [%] | 88.2 | 86.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 289 | 50mM Tris-HCl(pH8.5), 300mM MgCl2, 14% PEG2000, VAPOR DIFFUSION, SITTING DROP, temperature 289K |