3X1Y
Ras-related protein Rap1B(L9V) with GppNHp
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-17A |
Synchrotron site | Photon Factory |
Beamline | BL-17A |
Temperature [K] | 95 |
Detector technology | PIXEL |
Collection date | 2014-11-07 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.98000 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 43.600, 52.600, 60.720 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 35.416 - 1.168 |
R-factor | 0.1783 |
Rwork | 0.176 |
R-free | 0.21890 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.009 |
RMSD bond angle | 1.477 |
Data reduction software | MOSFLM |
Data scaling software | Aimless |
Phasing software | MOLREP |
Refinement software | PHENIX ((phenix.refine: dev_1412)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 35.420 | 1.190 |
High resolution limit [Å] | 1.168 | 1.170 |
Rmerge | 0.047 | 0.167 |
Number of reflections | 413294 | |
<I/σ(I)> | 23.3 | 8.9 |
Completeness [%] | 98.0 | 93.3 |
Redundancy | 8.8 | 7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298 | 0.1M HEPES pH 7.5, 12%(w/v) PEG3350, 5mM Cadmium chloride, VAPOR DIFFUSION, HANGING DROP, temperature 298K |