3X1D

Crystal Structure of Atlastin from Drosophila melanogaster

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	CCD
Collection date	2013-11-27
Detector	MAR CCD 165 mm
Wavelength(s)	1.54
Spacegroup name	C 1 2 1
Unit cell lengths	71.617, 63.907, 107.762
Unit cell angles	90.00, 101.60, 90.00

Refinement procedure

Resolution	27.793 - 2.870
R-factor	0.2783
Rwork	0.273
R-free	0.37850
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	Atlastin of human
RMSD bond length	0.009
RMSD bond angle	1.639
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASES
Refinement software	PHENIX ((phenix.refine: 1.8.2_1309))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	50.000
High resolution limit [Å]	2.870	2.870
Number of reflections	10190
Completeness [%]	35.0	35

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.4	293	20% PEG4000, 0.1M potassium phosphate, 0.2M magnesium chloride, 0.1M cacodylate, pH 7.4, VAPOR DIFFUSION, SITTING DROP, temperature 293K