3X0Y
Crystal structure of FMN-bound DszC from Rhodococcus erythropolis D-1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE AR-NW12A |
| Synchrotron site | Photon Factory |
| Beamline | AR-NW12A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-05-20 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 1.0000 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 145.480, 123.520, 184.520 |
| Unit cell angles | 90.00, 101.31, 90.00 |
Refinement procedure
| Resolution | 44.380 - 2.300 |
| R-factor | 0.23165 |
| Rwork | 0.230 |
| R-free | 0.27232 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.867 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.440 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Number of reflections | 140320 | |
| Completeness [%] | 99.4 | 96.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6 | 293 | 0.2M Malonate (pH 6.0), 24% (w/v) PEG 3350, 50mM NaF, 1mM FMN, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
