3X0X
Crystal structure of apo-DszC from Rhodococcus erythropolis D-1
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-5A |
Synchrotron site | Photon Factory |
Beamline | BL-5A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-04-15 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 1.0000 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 146.130, 123.250, 184.270 |
Unit cell angles | 90.00, 101.21, 90.00 |
Refinement procedure
Resolution | 43.910 - 2.110 |
R-factor | 0.18938 |
Rwork | 0.186 |
R-free | 0.25256 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.016 |
RMSD bond angle | 1.719 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | MOLREP |
Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.240 |
High resolution limit [Å] | 2.110 | 2.110 |
Number of reflections | 179463 | |
Completeness [%] | 98.2 | 95.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 6 | 293 | 0.2M Malonate (pH 6.0), 24% (w/v) PEG 3350, 50mM NaF, temperature 293K |