3X0I
ADP ribose pyrophosphatase in apo state at 0.91 angstrom resolution
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL38B1 |
Synchrotron site | SPring-8 |
Beamline | BL38B1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-10-23 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.7 |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 49.683, 49.683, 118.182 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 20.000 - 0.910 |
R-factor | 0.1359 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1v8r |
RMSD bond length | 0.021 |
RMSD bond angle | 2.620 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | MOLREP |
Refinement software | SHELXL-97 |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 20.000 | 20.000 | 0.940 |
High resolution limit [Å] | 0.910 | 1.960 | 0.910 |
Number of reflections | 119766 | ||
Completeness [%] | 98.3 | 99.1 | 96.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 298 | 0.24M Acetate-Sodium acetate buffer, 0.32M Ammonium sulfate, 30%(w/v) Glycerol, 8% (w/v) PEG 20000, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 298K |