3X0G

Crystal structure of the ectodomain of African green monkey CD81 large extracellular loop (agmCD81-LEL)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	100
Detector technology	CCD
Collection date	2014-06-27
Detector	RIGAKU SATURN 944+
Wavelength(s)	1.54
Spacegroup name	P 21 2 21
Unit cell lengths	39.156, 39.700, 59.915
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	25.276 - 1.899
R-factor	0.232
Rwork	0.231
R-free	0.25430
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4BKH
RMSD bond length	0.009
RMSD bond angle	1.163
Data reduction software	XDS (package)
Data scaling software	XDS (package)
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.7.3_928))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	27.878	2.010
High resolution limit [Å]	1.899	1.900
Rmerge	0.064	0.485
Number of reflections	7580
<I/σ(I)>	21.28	2.42
Completeness [%]	97.2	83.1
Redundancy	5.93	2.52

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	295	5% Ethanol, 5% MPD, 0.1M Tris pH 8.5, 200mM Sodium Chloride, VAPOR DIFFUSION, HANGING DROP, temperature 295K