3X0F
Crystal structure of the ectodomain of mouse CD81 large extracellular loop (mCD81-LEL)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-02-10 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 1.000020 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 44.617, 57.922, 62.080 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 36.231 - 1.471 |
| R-factor | 0.1997 |
| Rwork | 0.197 |
| R-free | 0.24370 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1g8q |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.629 |
| Data reduction software | XDS (package) |
| Data scaling software | XDS (package) |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.7.3_928)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.617 | 1.560 |
| High resolution limit [Å] | 1.471 | 1.470 |
| Rmerge | 0.023 | 0.469 |
| Number of reflections | 52310 | |
| <I/σ(I)> | 22.63 | 2.09 |
| Completeness [%] | 99.0 | 97.8 |
| Redundancy | 3.3 | 3.18 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 295 | 0.1M Sodium Citrate pH 5.5, 5% PEG 1000, 35% isopropanol, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
